Open AccessA transposable class I composite transposon carrying mph (methyl parathion hydrolase) from Pseudomonas sp. strain WBC‐3
Author(s) -
Wei Min,
Zhang JunJie,
Liu Hong,
Wang ShuJun,
Fu He,
Zhou NingYi
Publication year - 2009
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01468.x
Subject(s) - transposable element , biology , pseudomonas , parathion methyl , genetics , pseudomonas putida , strain (injury) , microbiology and biotechnology , gene , ralstonia , bacteria , mutant , pesticide , anatomy , agronomy
Pseudomonas sp. strain WBC‐3 utilizes methyl parathion ( O,O ‐dimethyl O ‐ p ‐nitrophenol phosphorothioate) or para ‐nitrophenol as the sole source of carbon, nitrogen and energy. A gene encoding methyl parathion hydrolase (MPH) had been characterized previously and found to be located on a typical class I composite transposon that comprised IS 6100 (Tn mph ). In this study, the transposability of this transposon was confirmed by transposition assays in two distinct mating‐out systems. Tn mph was demonstrated to transpose efficiently in a random manner in Pseudomonas putida PaW340 by Southern blot and in Ralstonia sp. U2 by sequence analysis of the Tn mph insertion sites, both exhibiting MPH activity. The linkage of the mph ‐like gene with IS 6100 , together with the transposability of Tn mph , as well as its capability to transpose in other phylogenetically divergent bacterial species, suggest that Tn mph may contribute to the wide distribution of mph ‐like genes and the adaptation of bacteria to organophosphorus compounds.