Open AccessTransposition of an insertion sequence, IS Tth7 , in the genome of the extreme thermophile Thermus thermophilus HB8
Author(s) -
Gregory Steven T.,
Dahlberg Albert E.
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01389.x
Subject(s) - thermus thermophilus , thermus , genetics , insertion sequence , biology , transposase , transposition (logic) , genome , whole genome sequencing , mobile genetic elements , circular bacterial chromosome , transposable element , gene , thermophile , escherichia coli , bacteria , linguistics , philosophy
We have identified an active insertion sequence (IS) in the genome of Thermus thermophilus HB8. Transposition was detected as insertional inactivation of a 16S rRNA methyltransferase gene, rsmG , resulting in streptomycin resistance. The IS element, IS Tth7 , is 1029 bp in length, encodes an imperfect 12 bp inverted repeat, and produces a 9 bp direct repeat of the target sequence. The sequence of a putative transposase encoded by IS Tth7 indicates that it is a member of the IS 427 group within the IS 5 family of ISs. Nine intact copies and several partial copies were identified throughout the chromosome and the megaplasmid pTT27. IS Tth7 was also detected in T. thermophilus strain IB‐21 and Thermus igniterrae but not Thermus antranikianii , suggesting a widespread occurrence of IS Tth7 among Thermus spp.