Open AccessAgrobacterium tumefaciens iron superoxide dismutases have protective roles against singlet oxygen toxicity generated from illuminated Rose Bengal
Author(s) -
Saenkham Panatda,
Utamapongchai Supa,
Vattanaviboon Paiboon,
Mongkolsuk Skorn
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01382.x
Subject(s) - mutant , singlet oxygen , superoxide dismutase , rose bengal , agrobacterium tumefaciens , reactive oxygen species , biology , microbiology and biotechnology , biochemistry , chemistry , oxygen , oxidative stress , gene , genetics , transformation (genetics) , organic chemistry
Singlet oxygen is a highly reactive form of molecular oxygen that is harmful to biological systems. Here, the role of three iron‐containing superoxide dismutase ( sodB ) genes is clearly shown in protecting Agrobacterium tumefaciens against singlet oxygen toxicity. A sodBI mutant was more sensitive to singlet oxygen than both wild‐type bacteria and a double sodBII–sodBIII mutant strain. Moreover, a sodBI – sodBII double mutant had higher sensitivity to singlet oxygen than a single sodBI mutant, although the double mutant was comparable to a sodB null mutant. High‐level expression of sodBI and sodBII fully complemented the singlet oxygen hypersensitivity phenotype of the sodB null mutant, while high‐level expression of sodBIII encoding a periplasmic SOD only partially restored the phenotype. Taken together, our data suggest that SodBI and SodBII have novel protective roles against singlet oxygen toxicity through unknown mechanisms.