Open AccessA putative lipoprotein of Sphingomonas sp. strain A1 binds alginate rather than a lipid moiety
Author(s) -
He Jinshan,
Ochiai Akihito,
Fukuda Yasuki,
Hashimoto Wataru,
Murata Kousaku
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01354.x
Subject(s) - moiety , strain (injury) , cytoplasm , sphingomonas , biochemistry , cell , polysaccharide , chemistry , lipoprotein , cell wall , biology , stereochemistry , gene , cholesterol , 16s ribosomal rna , anatomy
Gram‐negative Sphingomonas sp. strain A1 accumulates alginate in the cell surface pit and directly incorporates the polysaccharide into its cytoplasm through a ‘superchannel’. A cell surface protein Algp7 (27 kDa) is inducibly expressed in the presence of alginate. Although the protein Algp7 was initially classified as a lipoprotein based on its primary structure, Algp7 purified from strain A1 cells did not possess a lipid moiety. Algp7 bound alginate efficiently at a neutral pH with a K d of 3.6 × 10 −8 M, suggesting that the cell surface protein contributed to accumulation of alginate in the pit.