Open AccessExtracellular laccase activity and transcript levels of putative laccase genes during removal of the xenoestrogen technical nonylphenol by the aquatic hyphomycete Clavariopsis aquatica
Author(s) -
Solé Magali,
Kellner Harald,
Brock Susanne,
Buscot François,
Schlosser Dietmar
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01333.x
Subject(s) - laccase , xenoestrogen , nonylphenol , extracellular , vanillic acid , bioconversion , biology , food science , chemistry , microbiology and biotechnology , biochemistry , botany , environmental chemistry , enzyme , genetics , fermentation , cancer , estrogen receptor , breast cancer
We investigated the influence of potential laccase inducers with environmental relevance on extracellular laccase activity and removal of the xenoestrogen technical nonylphenol (tNP) by the aquatic hyphomycete Clavariopsis aquatica . Concomitantly, we identified two putative laccase gene fragments ( lcc1 and lcc2 ) and have followed their expression during removal of tNP under different conditions. Our results indicate a significant effect of copper on extracellular laccase activity in supernatants of fungal cultures. Laccase activity was highest in the presence of copper when added together with vanillic acid, followed by copper when used alone. Only slight laccase activities were recorded in the presence of only vanillic acid, whereas in the absence of either compound laccase activities were negligible. Laccase activity was well correlated with the removal efficiency of tNP, indicating the involvement of laccase in tNP bioconversion. Overall, lcc2 was less expressed than lcc1 . The expression of lcc1 and lcc2 correlated only partially with the measured laccase activity, suggesting the existence of cell‐associated laccase fractions not detectable in fungal culture supernatants and/or the existence of additional laccase genes.