Open AccessHeterologous expression of a histone‐like protein from Streptococcus intermedius in Escherichia coli alters the nucleoid structure and inhibits the growth of E. coli
Author(s) -
Liu Dali,
Yumoto Hiromichi,
Murakami Keiji,
Hirota Katsuhiko,
Kayama Shizuo,
Taniguchi Tomonori,
Yamamoto Akitake,
Ono Tsuneko,
Matsuo Takashi,
Miyake Yoichiro
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01327.x
Subject(s) - nucleoid , escherichia coli , biology , plasmid , reporter gene , microbiology and biotechnology , transformation (genetics) , green fluorescent protein , gene , heterologous expression , gene expression , biochemistry , recombinant dna
Escherichia coli failed to survive after transformation with a Streptococcus intermedius histone‐like protein gene ( Si‐hlp ) and its promoter‐harbored plasmid. The promoter function of Si‐hlp in E. coli was determined using enhanced green fluorescence protein ( egfp ) gene as a reporter. The inhibitory effect of Si ‐HLP on E. coli viability was verified by a tetracycline‐inducible gene expression system. Further study suggested that Si ‐HLP may alter the bacterial nucleoid structure, leading to the growth inhibition of E. coli .