Open AccessMycoplasma penetrans under nutritional stress: influence on lipid and lipoprotein profiles and on the binding to and invasion of HeLa cells
Author(s) -
Zeiman Einat,
Tarshis Mark,
Rottem Shlomo
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01322.x
Subject(s) - hela , strain (injury) , biology , biochemistry , cholesterol , sterol , lipoprotein , cardiolipin , bovine serum albumin , mycoplasma , microbiology and biotechnology , membrane , in vitro , phospholipid , anatomy
By serial passages through media containing decreasing concentrations of horse serum, the sterol‐requiring Mycoplasma penetrans strain HF‐2 was adapted to grow in a serum‐free medium supplemented with bovine serum albumin, cholesterol and free fatty acids. Chemical analysis of membrane preparations obtained from the native and adapted strains revealed two major differences. (1) The polar lipid fraction of the native strain contains, in addition to the de novo ‐synthesized phospholipids, exogenous lipids incorporated unchanged from the growth medium, whereas exogenous lipids were not detected in the adapted strain. (2) Protein analyses of the native and adapted strains showed that upon adaptation, the 42‐kDa membrane lipoprotein, one of the two major lipoproteins of this organism, was missing. Studies on the adhesion to, and invasion of HeLa cells by the native and adapted strains revealed that whereas the adherence to HeLa cells of the adapted strain was almost the same as that of the native strain, the invasiveness of the adapted strain into HeLa cells was very low or nonexistent.