Open AccessLocalization of the ActIII actinorhodin polyketide ketoreductase to the cell wall
Author(s) -
Xu XinPing,
Wang ZhiJun,
Fan KeQiang,
Wang ShengLan,
Jia CuiJuan,
Han Hui,
Ramalingam Eswar,
Yang KeQian
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01289.x
Subject(s) - actinorhodin , polyketide , streptomyces coelicolor , biology , biochemistry , polyketide synthase , biosynthesis , enzyme , gene , mutant
Structurally diverse polyketides provide a rich reservoir of bioactive molecules. Actinorhodin, a model aromatic polyketide, is synthesized by minimal type II polyketide synthase and tailoring enzymes. The ActIII actinorhodin ketoreductase is a key tailoring enzyme in actinorhodin biosynthesis. With purified antibodies against actinorhodin polyketide synthase α subunit (KSα) and ketoreductase, we conducted systematic localization experiments of the two proteins in Streptomyces coelicolor subproteomes. The results support the membrane location of KSα and cell‐wall location of ketoreductase. Considering previous evidence that some other tailoring enzymes of actinorhodin biosynthesis may be located outside the cytoplasm, a picture is emerging of an extensive role for extracellular biochemistry in the synthesis of type II polyketide antibiotic.