Open AccessCharacterization of soluble complexes of the Shigella flexneri type III secretion system ATPase
Author(s) -
Johnson Steven,
Blocker Ariel
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01284.x
Subject(s) - shigella flexneri , secretion , shigella , atpase , cytoplasm , type three secretion system , chemistry , microbiology and biotechnology , biochemistry , biology , escherichia coli , enzyme , virulence , gene
The conserved ATPase of type III secretion systems is critical to the export of substrates through the apparatus. We present a characterization of the native T3SS ATPase, Spa47, from the cytoplasm of Shigella flexneri , demonstrating it to be in two distinct high‐molecular‐weight complexes with Spa33: MxiN and MxiK.