Open AccessIsolation and characterization of Deinococcus geothermalis T27, a slightly thermophilic and organic solvent‐tolerant bacterium able to survive in the presence of high concentrations of ethyl acetate
Author(s) -
Kongpol Ajiraporn,
Kato Junichi,
Vangnai Alisa S.
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01273.x
Subject(s) - ethyl acetate , solvent , bacteria , toluene , thermophile , strain (injury) , bioremediation , chemistry , fructose , nuclear chemistry , biology , chromatography , organic chemistry , genetics , anatomy
A solvent‐tolerant, slightly thermophilic bacterium was isolated at 45 °C in the presence of toluene vapor provided as the sole carbon source. Strain T27 was identified as Deinococcus geothermalis T27. It could tolerate high concentrations of solvent provided as a nonaqueous layer (5% and 20%, v/v) to a cell suspension and had a remarkable ability to tolerate a broad range of solvents having log P ow values ranging from 5.6 of n ‐decane to as low as 0.7 of ethyl acetate. It was also able to utilize some of the solvents tested as a growth substrate at 45 °C. The addition of Ca 2+ ion, glucose and fructose partially promoted solvent tolerance. Cells exposed to ethyl acetate appeared to have a smaller size; however, the cell structure was not altered and was apparently well defined even after solvent shock. The tolerance of D. geothermalis T27 in the presence of high levels of toxic solvent stress at a comparatively high temperature indicated its potential use in biotechnological applications as well as bioremediation of xenobiotics.