Open AccessExpression of chromate resistance genes from Shewanella sp. strain ANA‐3 in Escherichia coli
Author(s) -
AguilarBarajas Esther,
Paluscio Elyse,
Cervantes Carlos,
Rensing Christopher
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01220.x
Subject(s) - chromate conversion coating , plasmid , escherichia coli , microbiology and biotechnology , efflux , operon , biology , gene , enterobacteriaceae , pseudomonas aeruginosa , gene expression , chemistry , bacteria , genetics , chromium , organic chemistry
The plasmidic chromate resistance genes chrBAC from Shewanella sp. strain ANA‐3 were transferred to Escherichia coli . Expression of chrA alone, on a high‐ or low‐copy number plasmid, conferred increased chromate resistance. In contrast, expression of the complete operon chrBAC on a high‐copy number plasmid did not result in a significant increase in resistance, although expression on a low‐copy number plasmid made the cells up to 10‐fold more resistant to chromate. The chrA gene also conferred increased chromate resistance when expressed in Pseudomonas aeruginosa . The chrR gene from the P. aeruginosa chromosome was necessary for full chromate resistance conferred by chrA . A diminished chromate uptake in cells expressing the chrA gene suggests that chromate resistance is due to chromate efflux.