Open AccessIsolation and characterization of Methanoculleus receptaculi sp. nov. from Shengli oil field, China
Author(s) -
Cheng Lei,
Qiu TianLei,
Li Xia,
Wang WeiDong,
Deng Yu,
Yin XiaoBo,
Zhang Hui
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01212.x
Subject(s) - biology , 16s ribosomal rna , strain (injury) , methanogenesis , thermophile , microbiology and biotechnology , bacteria , genetics , anatomy
Three strictly anaerobic, thermophilic methanogens (ZC‐2 T , ZC‐3 and ZC‐6) were isolated from Shengli oil field, China. The 16S rRNA gene sequences of the three strains were nearly identical, possessing >99.8% sequence similarity. They also possessed high sequence similarity, 97.4%, to Methanoculleus palmolei strain INSLUZ T (97.4% and 97.5%, respectively), indicating that they represented a novel species within the genus Methanoculleus . Cells of strain ZC‐2 T were nonmotile cocci, 0.8–1.7 μm in diameter, and always occurred singly or in pairs. The three strains used H 2 /CO 2 or sodium formate as substrates for methanogenesis but not sodium acetate, trimethylamine, monomethylamine, ethanol, dimethyl sulfide, isopropanol, isobutanol, butan‐2‐ol or H 2 /CO. Optimum growth of strain ZC‐2 T occurred in the presence of 0.2 M NaCl, pH 7.5–7.8 and temperature 50–55 °C with a specific growth rate of 0.084 h −1 . The mol% G+C content of the genomic DNA was 55.2 mol%. Based on these phenotypic and phylogenetic characteristics, strains ZC‐2 T , ZC‐3 and ZC‐6 are proposed to represent a novel species in the genus Methanoculleus and named Methanoculleus receptaculi sp. nov. The type strain is ZC‐2 T (CGMCC 1.5087 T =DSM 18860 T ).