Open AccessFilamentous vibriophage fs2 encoding the rst C gene integrates into the same chromosomal region as cholera toxin X
Author(s) -
Nguyen Dong Tu,
Nguyen Binh Minh,
Tran Huy Hoang,
Ngo Tuan Cuong,
Le Thanh Huong,
Nguyen Hoai Thu,
Albert M. John,
Iwami Mamoru,
Ehara Masahiko
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01200.x
Subject(s) - vibrio cholerae , genome , cholera toxin , gene , biology , chromosome , genetics , microbiology and biotechnology , bacteria
Abstract The genome of the filamentous phage of Vibrio cholerae fs2 was found to contain rst C and rst B1 (truncated) genes downstream of ORF500. att ‐fs2‐dir and att‐ fs2‐rev sequences homologous to that of att ‐CTXφ were found between orf 500 and rst C of the fs2 genome. This prompted us to search for the integration site of fs2 in the genomes of V. cholerae O1 and O139. The genome of fs2 was found to integrate downstream of att RS of the CTXφ phage, which integrated into chromosome I of V. cholerae O1 and O139. When infected with fs2, a fimbriate strain of V. cholerae O1 appeared to reduce fimbrial production in an adult rabbit ileal loop assay.