Open AccessHydrogen peroxide production by Lactobacillus johnsonii NCC 533 and its role in anti‐ Salmonella activity
Author(s) -
Pridmore Raymond David,
Pittet AnneCécile,
Praplan Fabienne,
Cavadini Christoph
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01176.x
Subject(s) - lactobacillus gasseri , salmonella enterica , microbiology and biotechnology , bacteriocin , antimicrobial , salmonella , biology , lactobacillus , hydrogen peroxide , probiotic , lactic acid , bacteria , pathogen , biochemistry , genetics
The human intestinal isolate Lactobacillus johnsonii NCC 533 (La1) is a probiotic strain with well‐documented antimicrobial properties. Previous research has identified the production of lactic acid and bacteriocins as important factors, but that other unidentified factors are also involved. We used the recently published genome sequence of L. johnsonii NCC 533 to search for novel antipathogen factors and identified three potential gene products that may catalyze the synthesis of the known antimicrobial factor hydrogen peroxide, H 2 O 2 . In this work, we confirmed the ability of NCC 533 as well as eight different L. johnsonii strains and Lactobacillus gasseri to produce H 2 O 2 when resting cells were incubated in the presence of oxygen, and that culture supernatant containing NCC 533‐produced H 2 O 2 was effective in killing the model pathogen Salmonella enterica serovar Typhimurium SL1344 in vitro .