Open AccessInteraction of enterohemorrhagic Escherichia coli O157:H7 with mouse intestinal mucosa
Author(s) -
Girard Francis,
Frankel Gad,
Phillips Alan D.,
Cooley William,
Weyer Ute,
Dugdale Alexandra H.A.,
Woodward Martin J.,
La Ragione Roberto M.
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01166.x
Subject(s) - escherichia coli , biology , secretion , microbiology and biotechnology , intestinal mucosa , epithelium , enterobacteriaceae , intestinal epithelium , effector , signal transducing adaptor protein , bacteria , lumen (anatomy) , virulence , biochemistry , gene , signal transduction , medicine , genetics
In this study, we used mouse ileal loops to investigate the interaction of enterohemorrhagic Escherichia coli (EHEC) O157:H7 with the mouse intestinal mucosa. With a dose of 10 9 and 3 h incubation, EHEC O157 was detected in the lumen and to a lesser extent associated with the epithelium. Typical attaching and effacing (A/E) lesions were seen, albeit infrequently. While the effector protein Tir was essential for A/E lesion formation, the bacterial type III secretion system adaptor protein TccP was dispensable. These results suggest that A/E lesions on mouse intestinal mucosa can be formed independently of robust actin polymerization.