Open AccessPrevotella intermedia induces matrix metalloproteinase‐9 expression in human periodontal ligament cells
Author(s) -
Guan SuMin,
Shu Lei,
Fu ShanMin,
Liu Bin,
Xu XiuLi,
Wu JunZheng
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01140.x
Subject(s) - prevotella intermedia , periodontal pathogen , matrix metalloproteinase , periodontal fiber , porphyromonas gingivalis , p38 mitogen activated protein kinases , mapk/erk pathway , kinase , periodontitis , extracellular matrix , chemistry , microbiology and biotechnology , biology , medicine , biochemistry , dentistry
Abstract Matrix metalloproteinases (MMPs) play pivotal roles in inflammatory diseases including chronic periodontitis. The effects of Prevotella intermedia , a major periodontal pathogen, on MMP‐9 production in primary human periodontal ligament (hPDL) cells were examined in the present study. MMP‐9 mRNA expression was measured by semiquantitative reverse transcriptase PCR and its protein secretion was assayed by gelatin zymography. Prevotella intermedia ATCC 25611 supernatant time and dose‐dependently induced MMP‐9 expression. In contrast, Porphyromanas gingivalis ATCC 33277 supernatants, Escherichia coli lipopolysacchride and IL‐1β exhibited no stimulatory effects on MMP‐9 production in hPDL cells. Mitogen‐activated protein kinases [MAPK, including extracellular signal‐related kinases (ERK), c‐jun N‐terminal kinases (JNK) and p38] inhibitors exerted no effect on the P. intermedia‐ induced MMP‐9 production, indicating that P. intermedia induced MMP‐9 production through an MAPK‐independent pathway. Our results demonstrated that P. intermedia may contribute to periodontal tissue destruction during chronic periodontitis by inducing MMP‐9 production in hPDL cells.