Open AccessStable low molecular weight RNA profiling showed variations within Sinorhizobium meliloti and Sinorhizobium medicae nodulating different legumes from the alfalfa cross‐inoculation group
Author(s) -
Del Villar María,
Rivas Raúl,
Peix Alvaro,
Mateos Pedro F.,
MartínezMolina Eustoquio,
Van Berkum Peter,
Willems Anne,
Velázquez Encarna
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01139.x
Subject(s) - sinorhizobium meliloti , sinorhizobium , biology , rhizobiaceae , inoculation , rna , microbiology and biotechnology , gene , nitrogen fixation , symbiosis , genetics , bacteria , immunology
Four different low molecular weight (LMW) RNA profiles, designated I–IV, among 179 isolates from Medicago , Melilotus and Trigonella species growing in a field site in Northern Spain were identified. From sequence analysis of the 16S rRNA, atpD and recA genes as well as DNA–DNA hybridization analysis with representatives of each LMW RNA profile it was evident that isolates with LMW RNA profiles I and II belonged to Sinorhizobium meliloti and those displaying profiles III and IV to Sinorhizobium medicae . Therefore, two distinct LMW RNA electrophoretic mobility profiles were found within each of these two species. Collectively, LMW RNA profiles I and II (identified as S. meliloti ) were predominant in Melilotus alba, Melilotus officinalis and Medicago sativa . Profiles III and IV (identified as S. medicae ) were predominant in Melilotus parviflora , Medicago sphaerocarpa , Medicago lupulina and Trigonella foenum‐graecum . All the four LMW RNA profiles were identified among isolates from Trigonella monspelliaca nodules. These results revealed a different specificity by the hosts of the alfalfa cross‐inoculation group towards the two bacterial species found in this study.