Open AccessInfluence of culture conditions on mycelial growth and bioluminescence of Gerronema viridilucens
Author(s) -
Mendes Luiz F.,
Bastos Erick L.,
Desjardin Dennis E.,
Stevani Cassius V.
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01118.x
Subject(s) - bioluminescence , petri dish , mycelium , agar , yeast extract , sugar , factorial experiment , biology , food science , yeast , chemistry , botany , microbiology and biotechnology , biochemistry , mathematics , fermentation , bacteria , statistics , genetics
Herein we describe a procedure for measuring the total light emission of the naturally bioluminescent tropical fungus Gerronema viridilucens and the optimization of culture conditions using multivariate factorial anova . Cultures growing on an agar surface in 35 mm Petri dishes at 90% humidity show optimal bioluminescence emission at 25 °C in the presence of 1.0% sugar cane molasses, 0.10% yeast extract and pH 6.0 (nonbuffered). Temperature and pH are the most important factors for both mycelial growth and bioluminescence.