Open AccessInhibitory effects of 7‐epiclusianone on glucan synthesis, acidogenicity and biofilm formation by Streptococcus mutans
Author(s) -
Murata Ramiro Mendonca,
Branco de Almeida Luciana Salles,
Yatsuda Regiane,
Dos Santos Marcelo Henrique,
Nagem Tanus Jorge,
Rosalen Pedro Luiz,
Koo Hyun
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01117.x
Subject(s) - streptococcus mutans , biofilm , extracellular , microbiology and biotechnology , glucan , intracellular , polysaccharide , glucosyltransferases , chemistry , biochemistry , bacteria , virulence , glucosyltransferase , enzyme , biology , genetics , gene
The aim of this study was to examine the effects of 7‐epiclusianone, a new prenylated benzophenone isolated from the plant Rheedia gardneriana , on some of the virulence properties of Streptococcus mutans associated with biofilm development and acidogenicity. The synthesis of glucans by glucosyltransferases B (GTF B) and C (GTF C) was markedly reduced by 7‐epiclusianone showing more than 80% inhibition of enzymatic activity at a concentration of 100 μg mL −1 . Double‐reciprocal analysis (Lineweaver–Burk plots) revealed that the inhibition of GTF B activity was noncompetitive (mixed) while GTF C was inhibited uncompetitively. The glycolytic pH drop by S. mutans cells was also disrupted by 7‐epiclusianone without affecting the bacterial viability, an effect that can be attributed, in part, to inhibition of F‐ATPase activity (61.1±3.0% inhibition at 100 μg mL −1 ). Furthermore, topical applications (1‐min exposure, twice daily) of 7‐epiclusianone (at 250 μg mL −1 ) disrupted biofilm formation and physiology. The biomass (dry‐weight), extracellular insoluble polysaccharide concentration and acidogenicity of the biofilms were significantly reduced by the test agent ( P <0.05). The data show that 7‐epiclusianone disrupts the extracellular and intracellular sugar metabolism of S. mutans , and holds promise as a novel, naturally occurring compound to prevent biofilm‐related oral diseases.