Open AccessThe Saccharomyces cerevisiae Wss1 protein is only present in mother cells
Author(s) -
Van Heusden G. Paul H.,
Steensma H. Yde
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01113.x
Subject(s) - saccharomyces cerevisiae , nucleolus , biology , subcellular localization , suppressor , green fluorescent protein , protein subcellular localization prediction , gene , microbiology and biotechnology , ubiquitin , nuclear membrane , nuclear protein , fusion protein , genetics , dna replication , mutation , cytoplasm , recombinant dna , transcription factor
The Saccharomyces cerevisiae WSS1 (Weak Suppressor of Smt3) gene has initially been identified as a multicopy suppressor of a mutation in SMT3 encoding the small ubiquitin‐like modifier. Later, multiple functions related to DNA replication and repair have been found for WSS1 . Here, we report the subcellular location of the Wss1 protein. Fluorescence microscopy of strains expressing a Wss1p‐green fluorescent protein (GFP) fusion shows that the protein is present in a single sharp spot near the nuclear membrane, distinct from the spindle pole bodies and nucleolus. In dividing cells, the spot is exclusively present in the mother cell, suggesting a mother cell‐specific function of WSS1 .