Open AccessAntibacterial activity of pyrrolidine dithiocarbamate
Author(s) -
Kang MiSun,
Choi EunKyoung,
Choi DoHyun,
Ryu SunYoul,
Lee HyeHyang,
Kang HoCheol,
Koh JeongTae,
Kim OkSu,
Hwang YunChan,
Yoon SukJa,
Kim SeonMi,
Yang KyuHo,
Kang InChol
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01069.x
Subject(s) - pyrrolidine dithiocarbamate , antibacterial activity , chemistry , staphylococcus aureus , antimicrobial , escherichia coli , zinc , broth microdilution , microbiology and biotechnology , chelation , bacteria , antibacterial agent , biochemistry , minimum inhibitory concentration , biology , antibiotics , organic chemistry , nf κb , apoptosis , gene , genetics
Pyrrolidine dithiocarbamate (PDTC), an antioxidant with a metal‐chelating activity, has been used widely to inhibit the expression of inflammatory genes in vitro and in vivo . This study investigated whether PDTC has an antimicrobial activity against various bacteria. The antibacterial activity of PDTC and other compounds was evaluated in vitro by the broth microdilution method against Porphyromonas gingivalis , Actinobacillus actinomycetemcomitans , Staphylococcus aureus , and Escherichia coli . Bacterial growth was inhibited by PDTC, where a wide range of sensitivity was demonstrated among the tested bacteria. The antibacterial activity of PDTC was reduced by the addition of copper chloride; in contrast, it was enhanced considerably by zinc chloride. Two different zinc chelators, Ca‐saturated EDTA (Ca‐EDTA) and N,N,N′,N′ ‐tetrakis (2‐pyridylmethyl) ethylenediamine, blocked the antibacterial activity of PDTC, whereas Zn‐EDTA failed to reduce the activity of PDTC. These results demonstrate for the first time that PDTC possesses an antibacterial activity, for which zinc is required, and suggest that PDTC, possessing a dual anti‐inflammatory and antibacterial activity, may be considered for topical use for inflammatory diseases of bacterial origin.