Open AccessRegulation of magnesium homeostasis in Salmonella : Mg 2+ targets the mgtA transcript for degradation by RNase E
Author(s) -
Spinelli Silvana V.,
Pontel Lucas B.,
García Véscovi Eleonora,
Soncini Fernando C.
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01065.x
Subject(s) - rnase p , magnesium , salmonella , homeostasis , chemistry , biology , microbiology and biotechnology , biochemistry , bacteria , genetics , rna , gene , organic chemistry
Extracellular Mg 2+ controls the activation of the Salmonella PhoP/PhoQ regulatory system. One of the adaptive responses driven by PhoP/PhoQ includes the transcriptional induction of mgtA and mgtCB , which encode two P‐type Mg 2+ transporters. Mg 2+ also controls mgtA expression by a riboswitch located in its 5′‐untranslated region (5′UTR). In this work, it was shown that the 5′UTR of both mgtA and mgtCB is responsible for a fine‐tuned Mg 2+ ‐dependent regulation of these genes. Evidence was also provided that the Mg 2+ riboswitch targets the mgtA transcript for degradation by RNase E when cells are grown in high Mg 2+ environments.