Open AccessDisruption of quinoprotein ethanol dehydrogenase gene and adjacent genes in Pseudomonas putida HK5
Author(s) -
Promden Worrawat,
Vangnai Alisa S.,
Pongsawasdi Piamsook,
Adachi Osao,
Matsushita Kazunobu,
Toyama Hirohide
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01060.x
Subject(s) - pseudomonas putida , alcohol dehydrogenase , mutant , gene , biology , gene cluster , pseudomonas , biochemistry , genetics , ethanol , bacteria
Pseudomonas putida HK5 produces three different quinoprotein alcohol dehydrogenases: ADH‐I, ADH‐IIB and ADH‐IIG. Gene organization of qedA , the gene for ADH‐I, and other 10 genes in the cluster was related to the genome sequences of five other Pseudomonas strains. Insertion mutations in either qedA , exaE or agmR eliminated ADH‐I activity, although the mutants were still able to grow on ethanol but more slowly than the wild‐type strain. Mutant analysis demonstrated the requirement of agmR and exaE in ADH‐I expression, and the tentative involvement of agmR , but not exaE , in the induction of ADH‐IIB and ADH‐IIG activities.