Open AccessIsolation and analysis of genes specifically expressed during basidiomatal development in Antrodia cinnamomea by subtractive PCR and cDNA microarray
Author(s) -
Chu FangHua,
Lee YiRu,
Chou ShuJen,
Chang TunTschu,
Shaw JeiFu
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2008.01052.x
Subject(s) - biology , complementary dna , gene , rapid amplification of cdna ends , sequence analysis , suppression subtractive hybridization , microbiology and biotechnology , homology (biology) , expressed sequence tag , cdna library , genetics , molecular cloning
cDNAs specifically expressed at the basidiome stage were isolated by using PCR‐selected cDNA subtraction in order to study gene regulation during porous‐hymenium basidiomatal formation in Antrodia cinnamomea . blastx results suggested that most of the expressed sequence tags (52.4–69.5%) had no significant protein homology to genes from other published living things. cDNAs particularly expressed at different growing conditions were identified using cDNA microarray analysis. Reverse transcriptase PCR analyses confirmed that the clone putative to P‐type ATPase, various cytochrome P450s and some unknown genes were abundant at natural basidiomes while endoglucanase was abundant at the tissue from artificial medium.