Open AccessProduction of biological active murine IFN‐γ by recombinant Lactococcus lactis
Author(s) -
BermúdezHumarán Luis G.,
CortesPerez Naima G.,
L'Haridon René,
Langella Philippe
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.01038.x
Subject(s) - lactococcus lactis , recombinant dna , vesicular stomatitis virus , biology , microbiology and biotechnology , bacteria , interferon gamma , genetically modified organism , virology , biological activity , virus , cytokine , lactic acid , biochemistry , in vitro , immunology , gene , genetics
IFN‐γ is a cytokine produced primarily by both T lymphocytes and natural killer cells and it is considered to be an attractive therapeutic molecule. In the present study, a DNA sequence encoding the mature murine IFN‐γ (muIFN‐γ) protein was cloned and expressed in the food‐grade lactic acid bacterium Lactococcus lactis . The activity of recombinant muIFN‐γ produced by genetically engineered L. lactis was confirmed in an antiviral assay using MoV cells infected with Vesicular Stomatitis Virus. The data provide the first demonstration that a Gram‐positive bacterium, L. lactis , is able to produce functional muIFN‐γ. This recombinant strain could lead to the development of a new, well‐tolerated vector to deliver active muIFN‐γ at the mucosal level.