Open AccessAssimilation of homotaurine‐nitrogen by Burkholderia sp. and excretion of sulfopropanoate
Author(s) -
Mayer Jutta,
Denger Karin,
Kaspar Katrin,
Hollemeyer Klaus,
Smits Theo H. M.,
Huhn Thomas,
Cook Alasdair M.
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.01014.x
Subject(s) - deamination , glutamate dehydrogenase , biochemistry , oxidative deamination , burkholderia , chemistry , carbon source , excretion , nitrogen , biology , glutamate receptor , bacteria , enzyme , organic chemistry , receptor , genetics
Homotaurine (3‐aminopropanesulfonate), free or derivatized, is in widespread pharmaceutical and laboratory use. Studies with enrichment cultures indicated that the compound is degradable as a sole source of carbon or as a sole source of nitrogen for bacterial growth. A pure culture of Burkholderia sp. was isolated which assimilated the amino group from homotaurine in a glucose–salts medium, and which released an organosulfonate, 3‐sulfopropanoate, into the medium stoichiometrically. The deamination involved an inducible 2‐oxoglutarate‐dependent aminotransferase to yield glutamate, and 3‐sulfopropanal. Release of the amino group was attributed to the measured NADP‐coupled glutamate dehydrogenase.