Open AccessGenetic elements for selection, deletion mutagenesis and complementation in Francisella spp.
Author(s) -
Ludu Jagjit S.,
Nix Eli B.,
Duplantis Barry N.,
De Bruin Olle M.,
Gallagher Larry A.,
Hawley Laura M.,
Nano Francis E.
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00979.x
Subject(s) - francisella , francisella tularensis , complementation , tularemia , plasmid , mutagenesis , biology , genetics , pathogen , selection (genetic algorithm) , mutant , gene , virology , virulence , computer science , artificial intelligence
Francisella novicida is a gram‐negative pathogen that can induce disease in mice that mimics human tularemia, and is nearly identical to Francisella tularensis at the genomic level. In this work a number of antibiotic marker cassettes that incorporate a strong F. novicida promoter is constructed, which greatly enhances selection in F. novicida and F. tularensis . Two low‐copy plasmid vectors based on a broad‐host‐range plasmid, and an integrating vector have also been made, and these can be used for genetic complementation. Two general approaches to deletion mutagenesis in F. novicida is also described.