Open AccessIncreased expression of laccase by the addition of phthalates in Phlebia tremellosa
Author(s) -
Yeo Sumin,
Kim Myung K.,
Choi Hyoung T.
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00971.x
Subject(s) - laccase , complementary dna , phthalic acid , trametes versicolor , chemistry , biochemistry , phthalate , rapid amplification of cdna ends , food science , enzyme , biology , molecular cloning , gene , organic chemistry
Phthalates, which are used as plasticizers in the plastics industry, are widely used and have been dispersed into the environment. A white‐rot basidiomycete Phlebia tremellosa , which showed good growth in media containing various hormone‐mimicking compounds, degraded benzylbutylphthalate and diethylphthalate by up to 30% and 80%, respectively, under liquid culture conditions for 9 days. A laccase cDNA from P. tremellosa was cloned by a rapid amplification of cDNA ends (RACE)‐PCR technique, and found to encode 1832 nucleotides. Its deduced amino acid sequence showed 80.7% identity when compared with that of Phlebia radiata , and 64.8% identity when compared with that of Trametes versicolor . When this fungus was grown under suitable conditions for degrading phthalic esters, the laccase activity and its transcript level were both highly increased.