Open AccessTranscript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli
Author(s) -
Baek Jong Hwan,
Kang Yeon Jae,
Lee Sang Yup
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00965.x
Subject(s) - escherichia coli , biology , phosphate , regulon , starvation , microbiology and biotechnology , biochemistry , gene , endocrinology
The responses to phosphate starvation by PhoR–PhoB two‐component regulatory system are also affected by other regulatory systems in Escherichia coli . The interactions among PhoB, PhoR, PhoU and CreC were investigated at transcript and protein levels using real‐time PCR and fluorescence resonance energy transfer analyses. CreC showed an interaction with PhoB, before an interaction between PhoB and PhoR, suggesting that this acts as a constant sensor for the early response to phosphate starvation. PhoU was found to interact with neither PhoB nor PhoR, while this acted as a phosphate starvation‐inducible protein. These results showed the detailed time‐ and phosphate concentration‐dependent interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in E. coli .