Open AccessSoluble and particulate methane monooxygenase gene clusters in the marine methanotroph Methylomicrobium sp. strain NI
Author(s) -
Nakamura Takamichi,
Hoaki Toshihiro,
Hanada Satoshi,
Maruyama Akihiko,
Kamagata Yoichi,
Fuse Hiroyuki
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00953.x
Subject(s) - methanotroph , methane monooxygenase , biology , gene , strain (injury) , genetics , microbiology and biotechnology , biochemistry , bacteria , anaerobic oxidation of methane , anatomy , catalysis
Soluble methane monooxygenase (sMMO) and particulate methane monooxygenase (pMMO) gene clusters in the marine methanotroph Methylomicrobium sp. strain NI were completely sequenced and analysed. Degenerated primers were newly designed and used to amplify the gene fragments containing intergenic mmoX–Y and mmoD–C regions and a partial pmoC region. Phylogenetic analysis of amino acid sequences deduced from mmoX and pmoA , as well as of 16S rRNA gene sequences, indicated that this strain was most closely related to the halotolerant methanotroph Methylomicrobium buryatense . There were putative σ 54 ‐ and σ 70 ‐dependent promoter sequences upstream of the sMMO and pMMO genes, respectively, and mmoG , which is known to be related to the expression and assembly of sMMO, existed downstream of the sMMO genes. These findings suggest that the major components and regulation of MMOs in this marine methanotroph are quite similar to those in freshwater methane oxidizers, despite the difference in their habitats.