Open AccessIdentification and characterization of variable‐number tandem‐repeat markers for the molecular epidemiological analysis of Mycoplasma mycoides subspecies mycoides SC
Author(s) -
McAuliffe Laura,
Ayling Roger D.,
Nicholas Robin A.J.
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00936.x
Subject(s) - variable number tandem repeat , minisatellite , biology , mycoplasma mycoides , genome , genetics , tandem repeat , subtyping , subspecies , microsatellite , allele , mycoplasma , gene , computer science , programming language , paleontology
Variable‐number tandem‐repeat (VNTR) analysis has been developed for the causative agent of contagious bovine pleuropneumonia using the genome of Mycoplasma mycoides subspecies mycoides small colony ( M.m.m . SC) PG1. Genome analysis identified 60 VNTRs within the M.m.m . SC genome; however, screening of these VNTRs with a panel of strains identified only three VNTRs that gave allelic variation. Testing of three VNTRs against 39 strains of diverse geographical origin gave 12 different VNTR profiles groups. VNTR analysis may represent a new rapid tool for subtyping M.m.m . SC isolates.