Open AccessEstablishment and characterization of dual‐species biofilms formed from a 3,5‐dinitrobenzoic‐degrading strain and bacteria with high biofilm‐forming capabilities
Author(s) -
Li Mengying,
Peng Lu,
Ji Zhang,
Xu Jingliang,
Li Shunpeng
Publication year - 2008
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00913.x
Subject(s) - biofilm , comamonas testosteroni , microbiology and biotechnology , bacteria , pseudomonas putida , achromobacter xylosoxidans , strain (injury) , bacillus cereus , chemistry , biology , pseudomonas , anatomy , genetics
In this study, the possibility of establishing a dual‐species biofilm from a bacterium with a high biofilm‐forming capability and a 3,5‐dinitrobenzoic acid (3,5‐DNBA)‐degrading bacterium, Comamonas testosteroni A3, was investigated. Our results showed that the combinations of strain A3 with each of five strains with a high biofilm‐forming capability ( Pseudomonas sp. M8, Pseudomonas putida M9, Bacillus cereus M19, Pseudomonas plecoglossicida M21 and Aeromonas hydrophila M22) presented different levels of enhancement regarding biofilm‐forming capability. Among these culture combinations, the 24‐h dual‐species biofilms established by C. testosteroni A3 with P. putida M9 and A. hydrophila M22 showed the strongest resistance to 3,5‐DNBA shock loading, as demonstrated by six successive replacements with DMM2 synthetic wastewater. The degradation rates of 3,5‐DNBA by these two culture combinations reached 63.3−91.6% and 70.7−89.4%, respectively, within 6 h of every replacement. Using the gfp ‐tagged strain M22 and confocal laser scanning microscopy, the immobilization of A3 cells in the dual‐species biofilm was confirmed. We thus demonstrated that, during wastewater treatment processes, it is possible to immobilize degrader bacteria with bacteria with a high biofilm‐forming capability and to enable them to develop into the mixed microbial flora. This may be a simple and economical method that represents a novel strategy for effective bioaugmentation.