Open AccessThe ptsP gene encoding the PTS family protein EI Ntr is essential for dimethyl sulfone utilization by Pseudomonas putida
Author(s) -
Kouzuma Atsushi,
Endoh Takayuki,
Omori Toshio,
Nojiri Hideaki,
Yamane Hisakazu,
Habe Hiroshi
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00882.x
Subject(s) - pseudomonas putida , organosulfur compounds , sulfone , dimethyl sulfoxide , dibenzothiophene , biochemistry , transposon mutagenesis , mutagenesis , chemistry , biology , gene , microbiology and biotechnology , mutant , sulfur , organic chemistry , transposable element
Many bacteria living in soil have developed the ability to use a wide variety of organosulfur compounds. Pseudomonas putida strain DS1 is able to utilize dimethyl sulfide as a sulfur source via a series of oxidation reactions that sequentially produce dimethyl sulfoxide, dimethyl sulfone (DMSO 2 ), methanesulfonate, and sulfite. To isolate novel genes involved in DMSO 2 utilization, a transposon‐based mutagenesis of DS1 was performed. Of c . 10 000 strains containing mini‐Tn 5 inserts, 11 mutants lacked the ability to utilize DMSO 2 , and their insertion sites were determined. In addition to the cysNC , cysH , and cysM genes involved in sulfate assimilation, the ptsP gene encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) family protein EI Ntr was identified, which is necessary for DMSO 2 utilization. Using quantitative reverse transcriptase‐polymerase chain reaction analysis, it was demonstrated that the expression of the sfn genes, necessary for DMSO 2 utilization, was impaired in the ptsP disruptant. To the authors' knowledge, this is the first report of a PTS protein that is involved in bacterial assimilation of organosulfur compounds.