Open AccessMultiple‐locus variable‐number tandem repeat analysis of Salmonella Enteritidis isolates from human and non‐human sources using a single multiplex PCR
Author(s) -
Cho Seongbeom,
Boxrud David J.,
Bartkus Joanne M.,
Whittam Thomas S.,
Saeed Mahdi
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00875.x
Subject(s) - multiple loci vntr analysis , variable number tandem repeat , salmonella enteritidis , biology , pulsed field gel electrophoresis , multiplex polymerase chain reaction , typing , tandem repeat , genetics , polymerase chain reaction , genotype , salmonella , genome , gene , bacteria
Simplified multiple‐locus variable‐number tandem repeat analysis (MLVA) was developed using one‐shot multiplex PCR for seven variable‐number tandem repeats (VNTR) markers with high diversity capacity. MLVA, phage typing, and PFGE methods were applied on 34 diverse Salmonella Enteritidis isolates from human and non‐human sources. MLVA detected allelic variations that helped to classify the S . Enteritidis isolates into more evenly distributed subtypes than other methods. MLVA‐based S . Enteritidis clonal groups were largely associated with sources of the isolates. Nei's diversity indices for polymorphism ranged from 0.25 to 0.70 for seven VNTR loci markers. Based on Simpson's and Shannon's diversity indices, MLVA had a higher discriminatory power than pulsed field gel electrophoresis (PFGE), phage typing, or multilocus enzyme electrophoresis. Therefore, MLVA may be used along with PFGE to enhance the effectiveness of the molecular epidemiologic investigation of S . Enteritidis infections.