Open AccessManganese affects the production of laccase in the basidiomycete Ceriporiopsis subvermispora
Author(s) -
Manubens Augusto,
Canessa Paulo,
Folch Carolina,
Avila Marcela,
Salas Loreto,
Vicuña Rafael
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00874.x
Subject(s) - laccase , mycelium , extracellular , manganese peroxidase , chemistry , enzyme , basidiomycota , biochemistry , xylanase , gene expression , food science , biology , gene , botany
The authors have previously identified and characterized lcs , a gene encoding laccase in the white‐rot basidiomycete Ceriporiopsis subvermispora . In this work, the effect of Mn 2+ in the production of extracellular laccase in liquid cultures of this fungus has been assessed. It was observed that at low (0–10 μM) concentrations of Mn 2+ , high titers of lcs ‐mRNA were obtained, whereas at high (160–194 μM) concentrations of this metal ion, transcripts levels decreased markedly. This phenomenon was observed at different days of growth. On the other hand, Cu 2+ or Ag + , but not Zn 2+ or Cd 2+ , led to an accumulation of lcs transcripts only in cultures grown in the absence of Mn 2+ . A dramatic increase in lcs transcript levels was also obtained with syringic acid, a lignin‐related aromatic compound. This effect was more pronounced in cultures lacking Mn 2+ . In the course of these studies it was observed that Mn 2+ stimulates mycelium growth. Thus, although extracellular laccase activity appeared higher in cultures containing 160 or 194 μM Mn 2+ , i.e. when lcs transcripts were lower, a correlation between lcs‐ mRNA levels and enzymatic activity was observed when values of the latter were corrected by the amount of mycelium present in the cultures.