Open AccessMembrane topology of the GltS Na + /glutamate permease of Escherichia coli
Author(s) -
Szvetnik Attila,
Gál József,
Kálmán Miklós
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00863.x
Subject(s) - permease , escherichia coli , symporter , membrane topology , topology (electrical circuits) , chemistry , biochemistry , biology , membrane , gene , membrane protein , transporter , mathematics , combinatorics
The GltS Na + /glutamate permease of Escherichia coli is the most extensively studied member of the ESS family of bacterial glutamate : Na + symporters. This paper presents the membrane topology analysis of the GltS with translational alkaline phosphatase and β‐galactosidase gene fusions generated by Tn phoA , nested deletions and targeted fusions. The topology model suggested by the translational fusion technique is compared with the MemGen model and discussed in detail.