Open AccessNew targets for the cyclic AMP receptor protein in the Escherichia coli K‐12 genome
Author(s) -
Hollands Kerry,
Busby Stephen J.W.,
Lloyd Georgina S.
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00826.x
Subject(s) - activator (genetics) , promoter , escherichia coli , camp receptor protein , biology , genome , transcription (linguistics) , genetics , receptor , transcription factor , regulator , dna binding protein , dna , microbiology and biotechnology , gene , gene expression , linguistics , philosophy
Recent genomic studies with Escherichia coli K‐12 have suggested scores of previously unexplored targets for the cyclic AMP receptor protein (CRP) global transcription regulator. Eleven of these loci were cloned and CRP binding was demonstrated at eight of these targets. It is shown that CRP can activate transcription at five of these targets and the functional DNA sites for CRP are identified. It is reported that CRP functions as a Class I activator at the aer promoter and as a Class II activator at the gatY , sdaC , ychH and malX promoters.