Open AccessAnalysis of interactions between nutrient germinant receptors and SpoVA proteins of Bacillus subtilis spores
Author(s) -
Vepachedu Venkata Ramana,
Setlow Peter
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00807.x
Subject(s) - bacillus subtilis , spore , receptor , operon , dipicolinic acid , spore germination , endospore , microbiology and biotechnology , biology , yeast , biochemistry , germination , sporogenesis , chemistry , gene , bacteria , genetics , botany , escherichia coli
Yeast two‐hybrid and Far Western analyses were used to detect interactions between Bacillus subtilis spores' nutrient germinant receptor proteins and proteins encoded by the spoVA operon, all of which are involved in spore germination and located in the spores' inner membrane. These analyses indicated that two subunits of the GerA nutrient germinant receptor interact, consistent with previous genetic data, and that some GerA proteins interact with SpoVAD and some with SpoVAE. SpoVA proteins appear to be involved in the release of the spore's dipicolinic acid during spore germination, an event triggered by the binding of nutrient germinants to their receptors. Consequently, these new findings suggest that nutrient germinant receptors physically contact SpoVA proteins, and presumably this is a route for signal transduction during spore germination.