Open AccessThe role of c‐di‐GMP signaling in an Aeromonas veronii biovar sobria strain
Author(s) -
Rahman Mokhlasur,
Simm Roger,
Kader Abdul,
Basseres Eugenie,
Römling Ute,
Möllby Roland
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00803.x
Subject(s) - biofilm , microbiology and biotechnology , quorum sensing , biovar , bacteria , aeromonas , pseudomonas aeruginosa , strain (injury) , chemistry , biology , motility , genetics , anatomy
Aeromonas is a ubiquitous gram‐negative bacterium that persists in the environment. It is shown that all isolates of persistent Aeromonas clones show strong biofilm formation ability. C‐di‐GMP regulates biofilm formation in many bacteria. To investigate the impact of c‐di‐GMP signaling, we introduced heterologous GGDEF and EAL domain proteins from Salmonella Typhimurium to an Aeromonas veronii biovar sobria strain. Overexpression of the GGDEF domain protein AdrA increased c‐di‐GMP concentration and biofilm formation and reduced motility. Production of the quorum‐sensing signaling molecule C4‐homoserine lactone and adhesion to aquatic plant duckweed and amoeba surfaces were enhanced. On the other hand, overexpression of the EAL domain protein YhjH decreased biofilm formation and increased motility.