Open AccessPorphyromonas gingivalis infection and cell death in human aortic endothelial cells
Author(s) -
Roth Georg A.,
Ankersmit Hendrik J.,
Brown Vinette B.,
Papapanou Panos N.,
Schmidt Ann Marie,
Lalla Evanthia
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00736.x
Subject(s) - porphyromonas gingivalis , endothelial stem cell , cell , microbiology and biotechnology , immunology , biology , medicine , periodontitis , biochemistry , in vitro
Porphyromonas gingivalis is a periodontal pathogen that promotes a proatherogenic response in endothelial cells. Cell death responses of human aortic endothelial cells to P. gingivalis at various multiplicities of infection (MOI) were investigated by assessment of cell detachment, histone‐associated DNA fragmentation, lactate dehydrogenase release and ADP:ATP ratio. Porphyromonas gingivalis at MOI 1 : 10–1 : 100 did not have a cytotoxic effect, but induced apoptotic cell death at MOI 1 : 500 and 1 : 1000. Monocyte chemoattractant protein‐1 production was significantly enhanced by P. gingivalis at MOI 1 : 100. At higher MOI, at least in vitro , P. gingivalis mediates endothelial apoptosis, thereby potentially amplifying proatherogenic mechanisms in the perturbed vasculature.