Open AccessIsolation and characterization of Bacillus thioparus sp. nov., chemolithoautotrophic, thiosulfate‐oxidizing bacterium
Author(s) -
PérezIbarra B. Mónica,
Flores María Elena,
GarcíaVarela Martín
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00729.x
Subject(s) - microbiology and biotechnology , thiosulfate , 16s ribosomal rna , biology , bacteria , strain (injury) , halotolerance , peptidoglycan , chemistry , sulfur , genetics , organic chemistry , anatomy
A novel bacterium, strain BMP‐1 T , was isolated from a continuous wastewater treatment culture system operating with a bacterial consortium. Cells of the isolate were Gram‐variable, aerobic, moderately halotolerant, motile and endospore‐forming rods. Strain BMP‐1 T grew chemolithoautotrophically by oxidation of thiosulfate to sulfate with a growth yield of 1.07 g protein mol −1 of thiosulfate consumed. DNA G+C content was 43.8 mol%. Its cell wall had peptidoglycan based on m ‐diaminopimelic acid, and the major component of fatty acid was C 15 : 0 . The 16S rRNA gene analysis showed that strain belongs to the genus Bacillus , sharing a 99.5% of sequence similarity with Bacillus jeotgali CCM 7133 T . DNA–DNA hybridization between the isolate of this study and this strain was 44%. Thus, the inclusion of strain BMP‐1 T in the genus Bacillus is suggested as a novel species and the name Bacillus thioparus sp. nov. (Type strain BMP‐1 T =BM‐B‐436 T =CECT 7196 T ) is proposed. The sequence of the 16S rRNA gene has been deposited in GenBank with accession number DQ371431 .