Open AccessMesorhizobium spp. are the main microsymbionts of Caragana spp. grown in Liaoning Province of China
Author(s) -
Yan Xue Rui,
Chen Wen Feng,
Fu Jun Fan,
Lu Yang Li,
Xue Cai Yun,
Sui Xin Hua,
Li Ying,
Wang En Tao,
Chen Wen Xin
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00727.x
Subject(s) - mesorhizobium , caragana , china , biology , botany , nitrogen fixation , geography , bacteria , rhizobia , archaeology , genetics
Caragana species are woody legumes widely distributed in the arid regions of China. These plants form root nodules but their nodule bacteria have not been clearly classified. A total of 112 symbiotic bacterial isolates were obtained from four Caragana species grown in Liaoning Province and were characterized with ARDRA, sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of proteins, BOX‐PCR and sequencing of the 16S rRNA gene. Most of them were classified as Mesorhizobium belonging to 11 putative species. Three isolates were identified as Rhizobium etli and Burkholderia spp. This study offers new information about the Caragana –rhizobia association and resource for selection of inoculants used in sustainable agriculture and for further studies on the Caragana rhizobia.