Open AccessIsolation and characterization of a carbofuran‐degrading strain Novosphingobium sp. FND‐3
Author(s) -
Yan QiuXiang,
Hong Qing,
Han Peng,
Dong XiaoJun,
Shen YuJia,
Li ShunPeng
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00718.x
Subject(s) - carbofuran , chemistry , metabolite , strain (injury) , hydroxylation , phenol , food science , pesticide , microbiology and biotechnology , stereochemistry , biology , organic chemistry , biochemistry , enzyme , anatomy , agronomy
A gram‐negative Novosphingobium sp. strain FND‐3 capable of degrading carbofuran was isolated and characterized. The carbofuran‐degrading ability of strain FND‐3 was investigated under various culture conditions. Strain FND‐3 showed a high average carbofuran‐degrading rate of 28.6 mg L −1 h −1 in mineral salts medium with 100 mg L −1 carbofuran. GC/MS analysis pointed out the presence of several unknown metabolites. One hydrolyzate was identified as 2‐hydroxy‐3‐(3‐methypropan‐2‐ol) phenol via hydrolysis of carbofuran phenol. The appearance of another metabolite with M + of 180 m / z indicated that the hydroxylation of carbofuran occurred at the aromatic ring. One novel degrading product with M + of 239 m / z was identified as 2‐hydroxy‐3‐(3‐methylpropan‐2‐ol) benzene‐ N ‐methylcarbamate via hydrolyzing at the ether bond of furanyl ring of carbofuran. Strain FND‐3 was also able to degrade other N ‐methylcarbamate pesticides.