Open AccessGenotypic comparison of five isolates of Rickettsia prowazekii by multilocus sequence typing
Author(s) -
Ge Hong,
Tong Min,
Jiang Ju,
Dasch Gregory A.,
Richards Allen L.
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00706.x
Subject(s) - rickettsia prowazekii , biology , typing , multilocus sequence typing , gene , genotype , genetics , sequence analysis , antigen , microbiology and biotechnology , virology , rickettsia , virus
Genetic traits of five Rickettsia prowazekii isolates, including the first from Africa and North America, and representatives from human and flying squirrels were compared using multilocus sequence typing. Four rickettsial genes encoding 17 kDa genus‐common antigen (17 kDa gene), citrate synthase ( gltA ), OmpB immunodominant antigen ( ompB ) and 120 kDa cytoplasmic antigen ( sca4 ) were examined. Sequence identities of 17 kDa gene and gltA were 100% among the isolates. Limited sequence diversity of ompB (0.02–0.11%) and sca4 (0.03–0.20%) was enough to distinguish the isolates, and evaluation of the combined four genes provided a method to easily differentiate R . prowazekii from other rickettsiae.