Open AccessProteomic analysis of Bacillus cereus growing in liquid soil organic matter
Author(s) -
Luo Yun,
Vilain Sébastien,
Voigt Birgit,
Albrecht Dirk,
Hecker Michael,
Brözel Volker S.
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00692.x
Subject(s) - bacillus cereus , biochemistry , chemistry , bacteria , staining , organic matter , chromatography , proteome , amino acid , nucleic acid , biology , organic chemistry , genetics
Bacillus cereus is believed to be a soil bacterium, but studied solely in laboratory culture media. The aim of this study was to assess the physiology of B. cereus growing on soil organic matter by a proteomic approach. Cells were cultured to mid‐exponential phase in soil extracted solubilized organic matter (SESOM), which mimics the nutrient composition of soil, and in Luria–Bertani broth as control. Silver staining of the two‐dimensional gels revealed 234 proteins spots up‐regulated when cells were growing in SESOM, with 201 protein spots down‐regulated. Forty‐three of these differentially expressed proteins were detected by Colloidal Coomassie staining and identified by matrix assisted laser desorption ionization–time of flight MS of tryptic digests. These differentially expressed proteins covered a range of functions, primarily amino acid, lipid, carbohydrate and nucleic acid metabolism. These results suggested growth on soil‐associated carbohydrates, fatty acids and/or amino acids, concomitant with shifts in cellular structure.