Open AccessEfficient downregulation of alb1 gene using an AMA1‐based episomal expression of RNAi construct in Aspergillus fumigatus
Author(s) -
Khalaj Vahid,
Eslami Hamid,
Azizi Mohammad,
RoviraGraells Nuria,
Bromley Mike
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00680.x
Subject(s) - downregulation and upregulation , aspergillus fumigatus , rna interference , gene silencing , biology , transformation (genetics) , gene , gene expression , small hairpin rna , microbiology and biotechnology , genetics , rna
An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus . Transformation of fungal protoplasts resulted in a high number of transformants with an inducible silenced phenotype (white spores). Efficient downregulation of the alb1 gene using this system suggests that this approach may overcome the variable downregulation observed with integrative constructs.