Open AccessEfficient silencing of the gene coding for the ɛ subunit of DNA polymerase III in Escherichia coli is triggered by antisense RNAs featuring stability in vivo
Author(s) -
Stefan Alessandra,
Tabler Martin,
Hochkoeppler Alejandro
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00679.x
Subject(s) - antisense rna , biology , microbiology and biotechnology , escherichia coli , in vivo , polymerase , gene expression , dna , gene , genetics
The Escherichia coli gene dnaQ coding for the ɛ subunit of DNA polymerase III was suppressed in vivo via antisense RNAs. To this aim, different fragments of dnaQ were cloned in reverse orientation into the pBAD‐HisB vector or into pT3T7, and the corresponding antisense RNAs were conditionally expressed in vivo . Antisense transcripts featuring fast hybridization in vitro with dnaQ mRNA but lacking stability in vivo did not suppress the target gene. Moreover, the in vivo concentration of an antisense transcript was positively correlated to its silencing effectiveness.