Open AccessHypervariable loci that enhance the discriminatory ability of newly proposed 15‐loci and 24‐loci variable‐number tandem repeat typing method on Mycobacterium tuberculosis strains predominated by the Beijing family
Author(s) -
Iwamoto Tomotada,
Yoshida Shiomi,
Suzuki Katsuhiro,
Tomita Motohisa,
Fujiyama Riyo,
Tanaka Noriko,
Kawakami Yasuto,
Ito Masahiro
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00658.x
Subject(s) - genetics , typing , biology , hypervariable region , mycobacterium tuberculosis , variable number tandem repeat , tandem repeat , beijing , tuberculosis , virology , genotype , gene , genome , medicine , geography , pathology , archaeology , china
Abstract The newly proposed 15‐ and 24‐loci mycobacterial interspersed repetitive unit (MIRU)–variable‐number tandem repeat (VNTR) typing method was evaluated for its ability to differentiate 181 Mycobacterium tuberculosis Beijing family strains. Compared with the original 12‐loci MIRU–VNTR typing method, the 15‐loci system dramatically improved the discriminatory power for Beijing strains; however, large clusters that could be further differentiated by IS 6110 restriction fragment length polymorphism (RFLP) were still obtained. The clonal stability and allelic diversity of a total of 31 VNTR loci were evaluated. VNTRs 3232, 3820, and 4120 were identified as the effective hypervariable VNTR set for the second‐line typing of clustered strains following the 15‐loci based scheme. Consequently, the discriminatory power of the new scheme (18 loci) equaled that of IS 6110 RFLP.