Open AccessPresence of a novel 16S–23S rRNA gene intergenic spacer insert in Bradyrhizobium canariense strains
Author(s) -
Safronova Vera,
Chizhevskaya Elena,
Bullitta Simonetta,
Andronov Evgeny,
Belimov Andrei,
Charles Trevor C.,
Lindström Kristina
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00623.x
Subject(s) - biology , amplified fragment length polymorphism , intergenic region , bradyrhizobium , genetics , 16s ribosomal rna , restriction fragment length polymorphism , gene cluster , spacer dna , gene , ribosomal rna , botany , rhizobium , internal transcribed spacer , genotype , genome , genetic diversity , population , demography , sociology
Seven slow‐growing bacterial strains isolated from root nodules of yellow serradella ( Ornithopus compressus ) that originated from Asinara Island on North Western Sardinia in Italy were characterized by partial 16S rRNA gene and intergenic spacer (ITS) sequencing as well as amplified fragment length polymorphism (AFLP) genomic fingerprinting. The results indicated that the O. compressus isolates belong to the Bradyrhizobium canariense species. The analysis of ITS sequences divided the branch of B. canariense strains into two statistically separated groups (ITS clusters I and II). All the strains in ITS cluster I showed the presence of unique oligonucleotide insert TTAGAGACTTAGGTTTCTK. This insert was neither found in other described species of the family Rhizobiaceae nor in any other bacterial families and can be used as a natural and high selective genetic marker for ITS cluster I of B. canariense strains. ITS grouping of O. compressus isolates was supported by the unweighted pair group method with arithmetic averages cluster analysis of their AFLP patterns, suggesting that the strains of ITS cluster II were genetically closer to each other than to isolates from the ITS cluster I. A taxonomic importance is supposed of the revealed 19 bp ITS insert for an intraspecific division within high heterogeneous B. canariense species.