Open AccessCharacterization and expression patterns of Sinorhizobium meliloti tmRNA ( ssrA )
Author(s) -
Ulvé Vincent M.,
Chéron Angélique,
Trautwetter Annie,
Fontenelle Catherine,
BarloyHubler Frédérique
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00616.x
Subject(s) - sinorhizobium meliloti , sinorhizobium , biology , genetics , microbiology and biotechnology , gene , rhizobiaceae , bacteria , symbiosis , mutant
tmRNA ( ssrA ) in Sinorhizobium meliloti is a small RNA annotated by homology with the Bradyrhizobium japonicum sra molecule. Here, this molecule is described in Sinorhizobium meliloti as a model for such molecules in Alphaproteobacteria subgroup‐2. Northern blot analysis and mapping of both 5′ and 3′ ends of this tmRNA allow the identification of two pieces: a 214 nt mRNA‐like domain and an 82 nt tRNA‐like domain, both highly stable, whereas the premature form is unstable. Transcriptional studies reveal that Sinorhizobium meliloti tmRNA is mainly expressed during growth resumption, replication initiation and various stress responses.